IREB2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IREB2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 23829-1-AP

Synonyme

ACO3, IRE BP 2, IREB2, Iron regulatory protein 2, IRP2, IRP2AD

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 23829-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 23829-1-AP (IREB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-13 using 23829-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 23829-1-AP (IREB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 23829-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23829-1-AP (IREB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 23829-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 23829-1-AP (IREB2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 23829-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 23829-1-AP (IREB2 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 23829-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23829-1-AP (IREB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 23829-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23829-1-AP (IREB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 23829-1-AP

  Immunofluorescent analysis of A549 cells using 23829-1-AP (IREB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, A549-Zellen, PC-13-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

23829-1-AP bindet in WB, IHC, IF, CoIP, ELISA IREB2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IREB2 fusion protein Ag20653
• Vollständiger Name: iron-responsive element binding protein 2
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 90-105 kDa
• GenBank-Zugangsnummer: BC017880
• Gene symbol: IREB2
• Gene ID (NCBI): 3658
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IREB2 is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner.

Publikationen

Species	Application	Title
mouse	WB	EMBO Rep; The deubiquitinase OTUD1 enhances iron transport and potentiates host antitumor immunity.; Authors - Jia Song
human	WB	Cell Mol Life Sci; Phenazine derivatives attenuate the stemness of breast cancer cells through triggering ferroptosis.; Authors - Yue Yang
human	WB,IF,CoIP	Free Radic Biol Med; Iron regulatory protein 2 is required for artemether -mediated anti-hepatic fibrosis through ferroptosis pathway.; Authors - Yujia Li ; KD Validated
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Iron deposition-induced ferroptosis in alveolar type II cells promotes the development of pulmonary fibrosis.; Authors - Haipeng Cheng
human	WB	Nutrients; Iron Transport from Ferrous Bisglycinate and Ferrous Sulfate in DMT1-Knockout Human Intestinal Caco-2 Cells.; Authors - Xiaonan Yu
mouse	WB,IF	Neurochem Int; Activation of vitamin D receptor inhibits Tau phosphorylation is associated with reduction of iron accumulation in APP/PS1 transgenic mice.; Authors - Ting-Yao Wu