Rabbit IgG control Polyklonaler Antikörper

Rabbit IgG control Polyklonal Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Anwendung

FC

Konjugation

CoraLite®594 Fluorescent Dye

Kat-Nr. : CL594-30000

Synonyme

IgG, IgG control, Isotype control, Rabbit IgG, Rabbit IgG isotype, Rabbit Immunoglobulin

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Galerie der Validierungsdaten

• 

  FC experiment of HeLa using CL594-30000

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated Rabbit IgG control (CL594-30000 (blue, solid line) or 0.4 ug CoraLite®594 Anti-Galectin-3 (CL594-14979) (red), or not stained (blank) (blue, dashed line). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

Erfolgreiche Detektion in FC

Empfohlene Verdünnung

• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-30000 bindet in FC Rabbit IgG control und zeigt Reaktivität mit

• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: Rabbit IgG control
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: N/A
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C