FC experiment of mouse bone marrow cells using CLV510-98136
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite®500 Rabbit IgG Isotype Control RecAb (CL500-98136, Clone: 240953C9)(red in histogram), or 0.25 ug CoraLite®500 Anti-Mouse Ly-6G Rabbit Recombinant Antibody (CL500-98284, Clone: 242141B11) (green in histogram), or unstained (blue in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite®500 Rabbit IgG Isotype Control RecAb (CL500-98136, Clone: 240953C9)(red in histogram), or 0.25 ug CoraLite®500 Anti-Mouse Ly-6G Rabbit Recombinant Antibody (CL500-98284, Clone: 242141B11) (green in histogram), or unstained (blue in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
CLV510-98136 targets IgG Isotype Control in FC applications and shows reactivity with n/a samples.
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
Background Information
This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Protocols
Product Specific Protocols
FC protocol for CoraLux Violet 510 IgG Isotype Control antibody CLV510-98136
FC experiment of mouse bone marrow cells using CLV510-98136
1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite®500 Rabbit IgG Isotype Control RecAb (CL500-98136, Clone: 240953C9)(red in histogram), or 0.25 ug CoraLite®500 Anti-Mouse Ly-6G Rabbit Recombinant Antibody (CL500-98284, Clone: 242141B11) (green in histogram), or unstained (blue in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IgG Isotype Control Recombinant antibody
Catalog Number
CLV510-98136
Citations
-
Dilutions
Applications
FC
Reactivity
n/a
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
