Rabbit IgG control Polyklonaler Antikörper

Rabbit IgG control Polyklonal Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

n/a

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Kat-Nr. : CL647-30000

Synonyme

Rabbit IgG, Rabbit Immunoglobulin, Rabbit IgG isotype, Isotype control, IgG control

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  FC experiment of HeLa using CL647-30000

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Rabbit IgG control (CL647-30000) (red), or 0.2 ug CoraLite® Plus 647-conjugated TRAP1 Rabbit PolyAb (CL647-10325) (green), or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-30000 bindet in FC (Intra) Rabbit IgG control und zeigt Reaktivität mit n/a

• Getestete Reaktivität: n/a
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: Rabbit IgG control
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: N/A
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody. This antibody can be used as Rabbit IgG control in experiments.