MHC Class II (I-A/I-E) Monoklonaler Antikörper

MHC Class II (I-A/I-E) Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

M5/114.15.2

Kat-Nr. : CL488-65122

Synonyme

Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II



Geprüfte Anwendungen

Erfolgreiche Detektion in IFMaus-Splenozyten
Erfolgreiche Detektion in FCMaus-Splenozyten

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:200-1:800
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65122 bindet in IF, FC MHC Class II (I-A/I-E) und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Ratte / IgG2b, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen Aktivierte C57BL/6-Mausmilzzellen
Vollständiger Name histocompatibility 2, class II antigen A, alpha
GenBank-ZugangsnummerBC031711
Gene symbol H2-Aa
Gene ID (NCBI) 14960
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 MHC Class II (I-A/I-E) antibody CL488-65122Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen