FcZero-rAb™ PE Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) Rabbit IgG Recombinant Antibody

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  FC experiment of mouse splenocytes using PE-FcA65663

  1x10^6 mouse splenocytes were surface stained with APC Anti-Mouse CD3e (145-2C11), and 0.1 ug PE Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) Rabbit IgG RecAb (PE-FcA65663, Clone: M5/114.15.2) or 0.1 ug PE Rabbit IgG Isotype Control RecAb (PE-FcA98136, Clone: 240953C9). Cells were not fixed.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

MHC Class II (I-A/I-E) FcZero-rAb^TM Recombinant Antibody for FC

Cat No. PE-FcA65663

Clone No.M5/114.15.2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

H2Aa, Aalpha, H 2Aa, H2-Aa, Ia 1

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.1 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-FcA65663 targets MHC Class II (I-A/I-E) in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: histocompatibility 2, class II antigen A, alpha
• GenBank Accession Number: BC031711
• Gene Symbol: MHC Class II
• Gene ID (NCBI): 14960
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 antibody is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).