MSH2 Monoklonaler Antikörper

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• KD/KO Validated

MSH2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

2H5F5

Kat-Nr. : 60161-1-Ig

Synonyme

2H5F5, MSH 2

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  WB analysis of HeLa using 60161-1-Ig

  WB results of MSH2 (60161-1-Ig, 1:1000) antibody with si-Control and si-MSH2 transfected HeLa cells..

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  WB analysis using 60161-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60161-1-Ig (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 60161-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60161-1-Ig (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 60161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Ramos using 60161-1-Ig

  Immunohistochemical analysis of paraffin-embedded Ramos cells slide using 60161-1-Ig (MSH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 60161-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MSH2 antibody (60161-1-Ig, Clone: 2H5F5 ) at dilution of 1:2277 and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005).

• 

  IF Staining of A431 using 60161-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MSH2 antibody (60161-1-Ig, Clone: 2H5F5 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, HCT 116-Zellen, HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Ramos-Zellen; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

60161-1-Ig bindet in WB, IHC, IF/ICC, ELISA MSH2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MSH2 fusion protein Ag7835
• Vollständiger Name: mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC021566
• Gene symbol: MSH2
• Gene ID (NCBI): 4436
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.

Publikationen

Species	Application	Title
mouse	IHC	Cancer Immunol Res; Remodeling Chondroitin-6-Sulfate-Mediated Immune Exclusion Enhances Anti-PD-1 Response in Colorectal Cancer with Microsatellite Stability.; Authors - Qijing Wu
human	WB	Sci Rep; Cold atmospheric plasma stabilizes mismatch repair for effective, uniform treatment of diverse colorectal cancer cell types; Authors - Yuanyuan He
human	WB	Transl Res; Novel MSH2 and TSC2 variants in a Chinese family with Lynch syndrome and their synergistic impact in urothelial carcinoma; Authors - Mingyang Li