IgG1 Isotype Control Monoklonaler Antikörper

IgG1 Isotype Control Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Megathura crenulata

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F8D3

Kat-Nr. : CL488-66360-1

Synonyme

Kappa light chain, Mouse IgG1, Mouse IgG1 isotype control

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Galerie der Validierungsdaten

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  FC experiment of human PBMCs using CL488-66360-1

  1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite® Plus 488 Anti-IgG1 Isotype Control (CL488-66360-1, Clone:1F8D3) (red), CL488 Anti-Human CD35 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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  FC experiment of A431 using CL488-66360-1

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Mouse IgG1 isotype control (CL488-66360-1, Clone: 1F8D3) (red), or 0.4 ug CoraLite® Plus 488 Anti-Human FKBP8 (CL488-66690, Clone: 1C1G12) (green), or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs, A431-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66360-1 bindet in FC IgG1 Isotype Control und zeigt Reaktivität mit Megathura crenulata

• Getestete Reaktivität: Megathura crenulata
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: IgG1 Isotype Control
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).