Nephrin Monoklonaler Antikörper

Nephrin Monoklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

CloneNo.

1D11H8

Kat-Nr. : 66970-1-Ig

Synonyme

NPHS1, 1D11H8, CNF, NPHN, NPHS 1

---

• 

  IHC staining of human kidney using 66970-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66970-1-Ig (Nephrin antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66970-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66970-1-Ig (Nephrin antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 10 publications below

Produktinformation

66970-1-Ig bindet in WB, IHC, IF, ELISA Nephrin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Nephrin fusion protein Ag19273
• Vollständiger Name: nephrosis 1, congenital, Finnish type (nephrin)
• Berechnetes Molekulargewicht: 1241 aa, 135 kDa
• GenBank-Zugangsnummer: BC156935
• Gene symbol: Nephrin
• Gene ID (NCBI): 4868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).

Publikationen

Species	Application	Title
	WB	Phytomedicine; Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro; Authors - Zhongzhu Ai
	WB,IF	Phytomedicine; Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis; Authors - Daofei Song
	IF	Genomics; Effectiveness of a novel rat model of off-target PLA2R1 knockout to renal impairment; Authors - Bo Huang
human	WB	Mol Cell Endocrinol; Experimental study on renoprotective effect of intermedin on diabetic nephropathy.; Authors - Yanhong Wang
	WB	Exp Mol Pathol; Urinary exosomes from patients with diabetic kidney disease induced podocyte apoptosis via microRNA-145-5p/Srgap2 and the RhoA/ROCK pathway; Authors - Lulu Han
	WB,IHC,IF	Heliyon; QiDiTangShen granules alleviates diabetic nephropathy podocyte injury: A network pharmacology study and experimental validation in vivo and vitro; Authors - Fei Gao