NR2F6 Monoklonaler Antikörper

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• KD/KO Validated

NR2F6 Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

2H2D11

Kat-Nr. : 60117-1-Ig

Synonyme

EAR 2, EAR2, ERBAL2, NR2F6, V erbA related protein 2

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 60117-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60117-1-Ig (NR2F6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 60117-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60117-1-Ig (NR2F6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 60117-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60117-1-Ig (NR2F6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 60117-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60117-1-Ig(NR2F6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 60117-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60117-1-Ig(NR2F6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 60117-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60117-1-Ig(NR2F6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 60117-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 60117-1-Ig(NR2F6 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

60117-1-Ig bindet in WB, IHC, ELISA NR2F6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NR2F6 fusion protein Ag7614
• Vollständiger Name: nuclear receptor subfamily 2, group F, member 6
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC002669
• Gene symbol: NR2F6
• Gene ID (NCBI): 2063
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NR2F6 belongs to NR2F subfamily members, which have their roles in the regulation of neurogenesis, organogenesis, and cellular differentiation during embryonic development [PMID:15875024]. NR2F6 acts as transcription factor by binding to a TGACCT direct-repeat motif and has been established in controlling facets of central nervous system (CNS) [12690040]. It is a nuclear attenuator that directly interferes with DNA binding of NF-AT and, subsequently, transcriptional activity of the NF-AT-dependent IL-17 expression. Otherwise, NR2F6 negatively interfered with transcriptional cytokine responses and acted as a barrier against autoimmunity [PMID:18701084].

Publikationen

Species	Application	Title
human,mouse	WB	Sci Adv; Melanoma-intrinsic NR2F6 activity regulates antitumor immunity; Authors - Hyungsoo Kim
human	WB,IHC	Int J Mol Sci; NR2F6 Expression Correlates with Pelvic Lymph Node Metastasis and Poor Prognosis in Early-Stage Cervical Cancer.; Authors - Chunhao Niu
human	IHC	Int J Cancer; PMID: 30895619; Authors - Han Li ; KD Validated