Filter: AllWBIF/ICCAffinity

WB analysis using 87372-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87372-1-RR (OTX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IF Staining of Y79 using 87372-1-RR
Immunofluorescent analysis of (4% PFA) fixed Y79 cells using OTX2 antibody (87372-1-RR, Clone: 252762H3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Affinity of 87372-1-RR
Biolayer interferometry (BLl) kinetic assays of 87372-1-RR against Human OTX2 were performed. The affinity constant is 0.132 nM.

OTX2 Recombinant monoclonal antibody

OTX2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87372-1-RR

Clone No.252762H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Homeobox protein OTX2, MCOPS5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

Positive WB detected in	ARPE-19 cells, mouse retina tissue, rat retina tissue
Positive IF/ICC detected in	Y79 cells

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC	IF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

87372-1-RR targets OTX2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag4323 Product name: Recombinant human OTX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-297 aa of BC032579 Sequence: MMSYLKQPPYAVNGLSLTTSGMDLLHPSVGYPGPWASCPAATPRKQRRERTTFTRAQLDVLEALFAKTRYPDIFMREEVALKINLPESRVQVWFKNRRAKCRQQQQQQQNGGQNKVRPAKKKTSPAREVSSESGTSGQFTPPSSTSVPTIASSSAPVSIWSPASISPLSDPLSTSSSCMQRSYPMTYTQASGYSQGYAGSTSYFGGMDCGSYLTPMHHQLPGPGATLSPMGTNAVTSHLNQSPASLSTQGYGASSLGFNSTTDCLDYKDQTASWKLNFNADCLDYKDQTSSWKFQVL Predict reactive species
Full Name	orthodenticle homeobox 2
Calculated Molecular Weight	297 aa, 32 kDa
Observed Molecular Weight	31-35 kDa
GenBank Accession Number	BC032579
Gene Symbol	OTX2
Gene ID (NCBI)	5015
RRID	AB_3745491
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P32243
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.