PABPN1 Recombinant monoclonal antibody, PBS Only

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• PABPN1 Recombinant monoclonal antibody, PBS Only (87392-1-PBS)

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  WB analysis using 87392-1-RR (same clone as 87392-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87392-1-RR (PABPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87392-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 87392-1-RR (same clone as 87392-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PABPN1 antibody (87392-1-RR, Clone: 252794F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87392-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 87392-1-RR (same clone as 87392-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PABPN1 antibody (87392-1-RR, Clone: 252794F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87392-1-PBS in a different storage buffer formulation.

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  Affinity of 87392-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87382-2-RR against Human PABPN1 were performed. The affinity constant is 22.7 pM.

PABPN1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 87392-1-PBS

Clone No.252794F12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Nuclear poly(A)-binding protein 1, PAB2, PABII, PABP2, PABP-2

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Product Information

87392-1-PBS targets PABPN1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag27814

  Product name: Recombinant human PABPN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 94-299 aa of BC010939

  Sequence: GSQEEEEEPGLVEGDPGDGAIEDPELEAIKARVREMEEEAEKLKELQNEVEKQMNMSPPPGNAGPVIMSIEEKMEADARSIYVGNVDYGATAEELEAHFHGCGSVNRVTILCDKFSGHPKGFAYIEFSDKESVRTSLALDESLFRGRQIKVIPKRTNRPGISTTDRGFPRARYRARTTNYNSSRSRFYSGFNSRPRGRVYRGRARA

• Full Name: poly(A) binding protein, nuclear 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 50-56 kDa
• GenBank Accession Number: BC010939
• Gene Symbol: PABPN1
• Gene ID (NCBI): 8106
• RRID: AB_3745510
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q86U42
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Poly(A) Binding Protein Nuclear 1(PABPN1) is one of the isoforms of poly(A) binding protein (PABP) that locating to the nucleus in eukaryotic cells. And PABPN1 plays important role in mRNA stability by regulating of poly-A tail length, mRNA decay and proximal polyadenylation site (PAS) utilization at the 3'-UTR (PMID:23300856). Another report showed that PABPN1 shuttles between the nucleus and the cytoplasm to export poly(A) RNA from the nucleus (PMID:23601051). It is reported that PABPN1 is ubiquitously expressed in almost all tissues, however, mutations in PABPN1 causes Oculopharyngeal Muscular Dystrophy (OPMD) which affected in skeletal muscles specifically. Because of a mild reduction in PABPN1 levels is sufficient to induce muscle wasting (PMID:27152426). Acetylation, Methylation, Phosphoprotein are common post-translational modifications of PABPN1 protein. And 50-55 kDa bands have been reported (PMID: 29939290; 28361972).