PACS2-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PACS2-Specific Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 19508-1-AP

Synonyme

KIAA0602, PACS 2, PACS1 like protein, PACS1L, PACS2, PACS-2, PACS2-Specific

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 19508-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19508-1-AP (PACS2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 19508-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19508-1-AP (PACS2-Specific antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 19508-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19508-1-AP (PACS2-Specific antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MCF-7 using 19508-1-AP

  Immunofluorescent analysis of MCF-7 cells using 19508-1-AP (PACS2-Specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Produktinformation

19508-1-AP bindet in WB, IP, IHC, IF, ELISA PACS2-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: phosphofurin acidic cluster sorting protein 2
• Berechnetes Molekulargewicht: 98 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: NM_015197
• Gene symbol: PACS2
• Gene ID (NCBI): 23241
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PACS2, also named PACS1L or KIAA0602, is one of PACS (phosphofurin acidic cluster sorting) proteins which are one such family of multifunctional membrane traffic regulators that mediate organ homoeostasis and have important roles in diverse pathologies and disease states. PACS2 is a multifunctional sorting protein that controls the endoplasmic reticulum (ER)-mitochondria communication, ER homeostasis, and apoptosis. PACS2 may be involved in ion channel trafficking, directing acidic cluster-containing ion channels to distinct subcellular compartments.

Publikationen

Species	Application	Title
human	WB	Nature; Autophagosomes form at ER-mitochondria contact sites.; Authors - Hamasaki Maho M
mouse	WB	Circulation; Hyperglycemia-Driven Inhibition of AMP-Activated Protein Kinase α2 Induces Diabetic Cardiomyopathy by Promoting Mitochondria-Associated Endoplasmic Reticulum Membranes In Vivo.; Authors - Shengnan Wu
human	WB	EMBO J; MITOL prevents ER stress-induced apoptosis by IRE1α ubiquitylation at ER-mitochondria contact sites.; Authors - Keisuke Takeda ; KD Validated
human, mouse	WB, IF, IP	Nat Commun; The sorting protein PACS-2 promotes ErbB signalling by regulating recycling of the metalloproteinase ADAM17.; Authors - Sarah Louise Dombernowsky ; KD Validated
human	WB	EMBO J; MITOL prevents ER stress-induced apoptosis by IRE1α ubiquitylation at ER-mitochondria contact sites.; Authors - Keisuke Takeda ; KD Validated
human,mouse	WB,IF,IP	Diabetes; PACS-2 Ameliorates Tubular Injury by Facilitating Endoplasmic Reticulum-Mitochondria Contact and Mitophagy in Diabetic Nephropathy.; Authors - Chenrui Li