PAX3 Polyklonaler Antikörper

PAX3 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 21386-1-AP

Synonyme

CDHS, HUP2, paired box 3, Paired box protein Pax 3, PAX3, WS1

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Galerie der Validierungsdaten

• 

  WB analysis of mouse skin using 21386-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21386-1-AP (PAX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21386-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21386-1-AP (PAX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skin using 21386-1-AP

  IP Result of anti-PAX3 (IP:21386-1-AP, 4ug; Detection:21386-1-AP 1:500) with mouse skin tissue lysate 3200ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushautgewebe, HEK-293-Zellen
• Erfolgreiche IP: Maushautgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

21386-1-AP bindet in WB, IP, IHC,ELISA PAX3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: hamster
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAX3 fusion protein Ag16021
• Vollständiger Name: paired box 3
• Berechnetes Molekulargewicht: 484 aa, 53 kDa
• Beobachtetes Molekulargewicht: 56-60 kDa
• GenBank-Zugangsnummer: BC101301
• Gene symbol: PAX3
• Gene ID (NCBI): 5077
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAX3, a transcription factor and multifunctional regulatory protein, is normally expressed during embryonic development. In the nervous system, PAX3 is involved in neural tube closure, neural crest development, and peripheral neuron differentiation. In the present study, PAX3 was reported as a novel regulator of GFAP transcription, and the overexpression and suppression of PAX3 could inhibit and promote NSC differentiation, respectively. In muscle development, PAX3 ensures the survival of myogenic progenitor cells with Pax3-expressing progenitors giving rise to both skeletal and smooth muscle cells. PAX3 also has a well-established role in the development of melanocytes during embryogenesis, and has recently been characterized as a molecular switch in the mature melanocyte. Mutations in PAX3 can cause Waardenburg syndrome.

Publikationen

Species	Application	Title
hamster	IHC	Birth Defects Res; Cigarette smoke induced neural tube defects by down-regulating noggin expression.; Authors - Chuanhui Yin