RB1CC1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RB1CC1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Kat-Nr. : 10043-2-AP

Synonyme

RB1CC1

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 10043-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10043-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10043-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10043-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10043-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10043-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10043-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10043-2-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10043-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10043-2-AP (RB1CC1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 10043-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10043-2-AP (RB1CC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10043-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10043-2-AP (RB1CC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10043-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10043-2-AP (RB1CC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10043-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10043-2-AP (RB1CC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10043-2-AP

  Immunofluorescent analysis of Hela cells, using RB1CC1 antibody 10043-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Maushirngewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 17 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Produktinformation

10043-2-AP bindet in WB, IP, IHC, IF, ELISA RB1CC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: RB1-inducible coiled-coil 1
• Berechnetes Molekulargewicht: 1594 aa, 183 kDa
• Beobachtetes Molekulargewicht: 180-200 kDa
• GenBank-Zugangsnummer: BC017556
• Gene symbol: RB1CC1
• Gene ID (NCBI): 9821
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation[PMID:15968549 ]. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation [PMID: 19258318]. It is probably involved in the tumorigenesis of breast cancer[PMID: 14533007]. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene[PMID: 21602932]. The immunogen of this antibody is C-terminal 154aa of RB1CC1.

Publikationen

Species	Application	Title
mouse	WB	Cell; Noncanonical autophagy promotes the visual cycle.; Authors - Kim Ji-Young JY ; KD Validated
human	WB	Nat Immunol; Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms.; Authors - Michal P Wandel ; KD Validated
human	WB	Nat Cell Biol; mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6.; Authors - Nazio Francesca F
human	IF	Nat Cell Biol; ATM functions at the peroxisome to induce pexophagy in response to ROS.; Authors - Jiangwei Zhang
mouse	WB	Genes Dev; p62/SQSTM1 synergizes with autophagy for tumor growth in vivo.; Authors - Huijun Wei
human, mouse	WB, IF	Nat Struct Mol Biol; Interaction between FIP200 and ATG16L1 distinguishes ULK1 complex-dependent and -independent autophagy.; Authors - Noor Gammoh

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