Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, K-562 cells, MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23599-1-AP targets RPS25 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7890 Product name: Recombinant human RPS25 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-125 aa of BC004986 Sequence: MPPKDDKKKKDAGKSAKKDKDPVNKSGGKAKKKKWSKGKVRDKLNNLVLFDKATYDKLCKEVPNYKLITPAVVSERLKIRGSLARAALQELLSKGLIKLVSKHRAQVIYTRNTKGGDAPAAGEDA Predict reactive species |
| Full Name | ribosomal protein S25 |
| Calculated Molecular Weight | 14 kDa |
| Observed Molecular Weight | 15-17 kDa |
| GenBank Accession Number | BC004986 |
| Gene Symbol | RPS25 |
| Gene ID (NCBI) | 6230 |
| RRID | AB_11183757 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62851 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ribosomal protein S25 (RPS25) is a novel MDM2 interacting protein. It may be involved in viral replication of Dicistroviridae and hepatitis C viruses. RPS25 is overexpressed in human leukemia cells exhibiting adriamycin resistance. It may have a role in p53-mediated apoptosis and cell-cycle arrest. The expected MW of RPS25 is about 15-17kDa. Catalog# 23599-1-AP is a rabbit polyclonal antibody raised against full-length RPS25, and is recommended for ELISA, Western blotting.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPS25 antibody 23599-1-AP | Download protocol |
| IHC protocol for RPS25 antibody 23599-1-AP | Download protocol |
| WB protocol for RPS25 antibody 23599-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPS25 Polyclonal antibody (Cat# 23599-1-AP) has 3 citations. They appear in ACS Nano, J Transl Med, and Mol Cell Proteomics. Research fields span bone regeneration via the GOLPH2-AKT signaling axis, paternal ribosome biogenesis dysfunction and early embryonic development, and quantitative proteomics of primary lung adenocarcinoma biomarkers. All citations used the antibody for Western blot in human or mouse samples.
| Species | Application | Title |
|---|---|---|
ACS Nano Platelet-Derived Apoptotic Vesicles Promote Bone Regeneration via Golgi Phosphoprotein 2 (GOLPH2)-AKT Signaling Axis | ||
J Transl Med Paternal ribosome biogenesis dysfunction caused by cyclophosphamide-busulfan chemotherapy contributes to early embryonic developmental impairment in mice. | ||
Mol Cell Proteomics Identification and characterization of potential biomarkers by quantitative tissue proteomics of primary lung adenocarcinoma. |
Reviews
What customers say
The single review for this antibody (rated 4/5) reports successful use in Western Blot at a 1:2000 dilution on HCT116 cells for CRISPR knock-in screening. The user found the antibody worked well overall but noted a couple of non-specific bands appearing above the target band.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
H (Verified Customer) (07-09-2020) | This antibody works well for screening of crispr knock-in. There were a couple non-specific bands above though.
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