SHMT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SHMT1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Rezensionen (1)

Kat-Nr. : 14149-1-AP

Synonyme

CSHMT, Serine methylase, SHMT, SHMT1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 14149-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14149-1-AP (SHMT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14149-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14149-1-AP (SHMT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 14149-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14149-1-AP (SHMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14149-1-AP

  Immunofluorescent analysis of HepG2 cells using 14149-1-AP (SHMT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below

Produktinformation

14149-1-AP bindet in WB, IHC, IF, ELISA SHMT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SHMT1 fusion protein Ag5330
• Vollständiger Name: serine hydroxymethyltransferase 1 (soluble)
• Berechnetes Molekulargewicht: 483 aa, 53 kDa
• Beobachtetes Molekulargewicht: 45-53 kDa
• GenBank-Zugangsnummer: BC038598
• Gene symbol: SHMT1
• Gene ID (NCBI): 6470
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SHMT1 is the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. This antibody can detect both 49 kDa and 53 kDa isoforms in western blotting.

Publikationen

Species	Application	Title
		Science; mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.; Authors - Issam Ben-Sahra
mouse	WB	Cell Metab; PMID: 28111214; Authors - Eric H Ma
human	WB	Nat Commun; Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.; Authors - Eduardo Balsa
human	WB	PLoS Pathog; SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.; Authors - Muyu Xu ; KD Validated
human	WB	Sci Rep; Vascular endothelial growth factor signaling requires glycine to promote angiogenesis.; Authors - Dongqing Guo
human	IF	J Cell Sci; Rod and Ring formation from IMP dehydrogenase is regulated via the one-carbon metabolic pathway.; Authors - S John Calise

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (03-26-2021)

Good Antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse liver, mouse brain / Hella, HepG2

SHMT1 Antibody Western Blot validation (1:2000 dilution) in mouse liver, mouse brain / Hella, HepG2 (Cat no:14149-1-AP)