TREM2 Polyklonaler Antikörper

TREM2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Kat-Nr. : 27599-1-AP

Synonyme

Trem2c, Trem2b, Trem2a, TREM-2, TREM 2

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  WB analysis using 27599-1-AP

  Non-treated and PMA treated THP-1 cellls were subjected to SDS PAGE followed by western blot with 27599-1-AP (TREM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

27599-1-AP bindet in WB, ELISA TREM2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TREM2 fusion protein Ag26319
• Vollständiger Name: triggering receptor expressed on myeloid cells 2
• Berechnetes Molekulargewicht: 222 aa, 25 kDa
• Beobachtetes Molekulargewicht: 26-32 kDa
• GenBank-Zugangsnummer: BC032362
• Gene symbol: TREM2
• Gene ID (NCBI): 54209
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213).

Publikationen

Species	Application	Title
human		Dev Cell; HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis; Authors - Xiang Peng
human	IF	Cell Death Dis; Heterogeneity of tumor microenvironment is associated with clinical prognosis of non-clear cell renal cell carcinoma: a single-cell genomics study.; Authors - Wen-Jin Chen
human,mouse	WB	Sci Adv; TREM2 inhibition triggers antitumor cell activity of myeloid cells in glioblastoma; Authors - Rui Sun
human	IF	Front Immunol; Identification of TREM2-positive tumor-associated macrophages in esophageal squamous cell carcinoma: implication for poor prognosis and immunotherapy modulation; Authors - Hongmu Li
human,mouse	WB,IHC,IF	Nat Commun; Soluble TREM2 ameliorates tau phosphorylation and cognitive deficits through activating transgelin-2 in Alzheimer's disease; Authors - Xingyu Zhang
mouse	WB	Brain Res; TREM2 regulates BV2 microglia activation and influences corticosterone-induced neuroinflammation in depressive disorders; Authors - Jingjing Shi