TREM2 Polyklonaler Antikörper

TREM2 Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 27599-1-AP

Synonyme

TREM 2, TREM2, Trem2a, Trem2b, Trem2c

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Galerie der Validierungsdaten

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  WB analysis using 27599-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27599-1-AP (TREM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of BV-2 using 27599-1-AP

  1X10^6 BV-2 cells were intracellularly stained with 0.4 ug Anti-Human TREM2 (27599-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MKN-45-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in FC: BV-2 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

27599-1-AP bindet in WB, IHC, IF, FC, ELISA TREM2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TREM2 fusion protein Ag26319
• Vollständiger Name: triggering receptor expressed on myeloid cells 2
• Berechnetes Molekulargewicht: 222 aa, 25 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC032362
• Gene symbol: TREM2
• Gene ID (NCBI): 54209
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213).

Publikationen

Species	Application	Title
human	IF	Cell Death Dis; Heterogeneity of tumor microenvironment is associated with clinical prognosis of non-clear cell renal cell carcinoma: a single-cell genomics study.; Authors - Wen-Jin Chen
human,mouse	WB	Sci Adv; TREM2 inhibition triggers antitumor cell activity of myeloid cells in glioblastoma; Authors - Rui Sun
human	IF	Front Immunol; Identification of TREM2-positive tumor-associated macrophages in esophageal squamous cell carcinoma: implication for poor prognosis and immunotherapy modulation; Authors - Hongmu Li
human,mouse	WB,IHC,IF	Nat Commun; Soluble TREM2 ameliorates tau phosphorylation and cognitive deficits through activating transgelin-2 in Alzheimer's disease; Authors - Xingyu Zhang
mouse	WB	Brain Res; TREM2 regulates BV2 microglia activation and influences corticosterone-induced neuroinflammation in depressive disorders; Authors - Jingjing Shi