TRPV1 Polyklonaler Antikörper

TRPV1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 22686-1-AP

Synonyme

Capsaicin receptor, Osm 9 like TRP channel 1, OTRPC1, TRPV1, Vanilloid receptor 1, VR1

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Galerie der Validierungsdaten

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  WB analysis of PC-12 using 22686-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 22686-1-AP (TRPV1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 22686-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22686-1-AP (TRPV1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 22686-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22686-1-AP (TRPV1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse skin using 22686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 22686-1-AP (TRPV1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse skin using 22686-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 22686-1-AP (TRPV1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 22686-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 22686-1-AP (TRPV1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 22686-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 22686-1-AP (TRPV1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautgewebe, humanes Kolongewebe, Maushautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

22686-1-AP bindet in WB, IHC, IF, ELISA TRPV1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TRPV1 fusion protein Ag18583
• Vollständiger Name: transient receptor potential cation channel, subfamily V, member 1
• Berechnetes Molekulargewicht: 839 aa, 95 kDa
• Beobachtetes Molekulargewicht: 95 kDa
• GenBank-Zugangsnummer: BC132820
• Gene symbol: TRPV1
• Gene ID (NCBI): 7442
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRPV1, also named as VR1 or OTRPC1, belongs to the transient receptor potential (TRP) family and TRPV subfamily (PMID: 17579562). TRPV1 is a ligand-activated non-selective calcium permeant cation channel expressed predominantly by sensory neurons (PMID: 10821274). It is involved in detection of noxious chemical and thermal stimuli. TRPV1 plays an important role in inflammatory thermal hyperalgesia (PMID: 10764638; 10821274).

Publikationen

Species	Application	Title
human	WB	Acta Pharmacol Sin; PMID: 29188798; Authors - Su-Shan Zhang
mouse	WB	J Neurosci; Phosphorylation of TRPV1 S801 Contributes to Modality-Specific Hyperalgesia in Mice.; Authors - John Joseph
mouse	IHC	Oncotarget; Dietary capsaicin and antibiotics act synergistically to reduce non-alcoholic fatty liver disease induced by high fat diet in mice.; Authors - Jingjuan Hu
rat	WB	Biomed Pharmacother; Suhuang antitussive capsule inhibits NLRP3 inflammasome activation and ameliorates pulmonary dysfunction via suppression of endoplasmic reticulum stress in cough variant asthma.; Authors - Weiwei Qin
mouse	WB	Biomed Pharmacother; Qingfei oral liquid alleviates airway hyperresponsiveness and mucus hypersecretion via TRPV1 signaling in RSV-infected asthmatic mice.; Authors - Xiaoping Jing
mouse	WB	Eur J Pharmacol; Spinophilin negatively controlled the function of transient receptor potential vanilloid 1 in dorsal root ganglia neurons of mice.; Authors - Zhen Guo

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