VEGFA Polyklonaler Antikörper

VEGFA Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 26381-1-AP

Synonyme

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF

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Galerie der Validierungsdaten

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  WB analysis using 26381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26381-1-AP (VEGFA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 26381-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26381-1-AP (VEGFA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26381-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26381-1-AP (VEGFA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 26381-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26381-1-AP (VEGFA Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 26381-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26381-1-AP (VEGFA Antibody) at dilution of 1:200 (under 40x lens).

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  FC experiment of NIH/3T3 using 26381-1-AP

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human VEGFA (26381-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

26381-1-AP bindet in WB, IHC, FC, CoIP, ELISA VEGFA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VEGFA fusion protein Ag24740
• Vollständiger Name: vascular endothelial growth factor A
• Berechnetes Molekulargewicht: 412 aa, 46 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC065522
• Gene symbol: VEGFA
• Gene ID (NCBI): 7422
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38 kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ).

Publikationen

Species	Application	Title
human	WB	Antioxidants (Basel); Suppression of NRF2 Activity by HIF-1α Promotes Fibrosis after Ischemic Acute Kidney Injury; Authors - Corry D Bondi
human	WB,CoIP, IHC	J Cell Mol Med; Piezo type mechanosensitive ion channel component 1 facilitates gastric cancer omentum metastasis.; Authors - Xiaofei Wang
human	WB,IHC	Oncol Lett; Mitochondrial calcium uniporter promotes cell proliferation and migration in esophageal cancer.; Authors - Yu Miao
human	WB	Cell Death Discov; Circ_0030998 promotes tumor proliferation and angiogenesis by sponging miR-567 to regulate VEGFA in colorectal cancer.; Authors - Longyang Jin