Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, MCF-7 cells, A431 cells |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28689-1-AP targets WWP1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30161 Product name: Recombinant human WWP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC036065 Sequence: MATASPRSDTSNNHSGRLQLQVTVSSAKLKRKKNWFGTAIYTEVVVDGEITKTAKSSSSSNPKWDEQLTVNVTPQTTLEFQVWSHRTLKADALLGKATIDLKQALLIHNRKLERVKEQLKLSLENKNGIAQTGELTVVLDGLVIEQENITNCSSSPTIEIQENGDALHENGEPSARTTARLAVEGTNGIDNHVPTSTLVQNSCCSYVVNGDNTPSSPSQVAARPKNTPAPKPLASEPADDTVNGESSSFAPTDNASVTGTPVVSEENALSPNCTSTTVEDPPVQEILTSSENNECIPSTSAELESEARSILEPDTSNSRSSSAFEAAKSRQPDGCMDPVRQQSGNANTET Predict reactive species |
| Full Name | WW domain containing E3 ubiquitin protein ligase 1 |
| Calculated Molecular Weight | 922 aa, 105 kDa |
| Observed Molecular Weight | 95-105 kDa |
| GenBank Accession Number | BC036065 |
| Gene Symbol | WWP1 |
| Gene ID (NCBI) | 11059 |
| RRID | AB_2881195 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H0M0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) is a multifunction protein containing an N-terminal C2 domain, four tandem WW domains for substrate binding, and a C-terminal catalytic HECT domain for ubiquitin transferring. It is also named as AIP5, Tiul1. WWP1 regulates a variety of cellular biological processes including protein trafficking and degradation, signaling, transcription, and viral budding. WWP1 has been implicated in several diseases, such as cancers, infectious diseases, neurological diseases, and aging(PMID:22051607). It has 6 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for WWP1 antibody 28689-1-AP | Download protocol |
| WB protocol for WWP1 antibody 28689-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The WWP1 Polyclonal antibody (Cat# 28689-1-AP) has 6 citations published in Drug Resist Updat, Cell Mol Biol Lett, Free Radic Biol Med, J Biochem Mol Toxicol, Cancer Med, and Mol Med. Research fields span ferroptosis, ubiquitination, autophagy, and proteasomal degradation, with applications in non-small cell lung cancer, prostate cancer, hepatocellular carcinoma, small cell lung cancer, and hyperuricemic nephropathy.
| Species | Application | Title |
|---|---|---|
Drug Resist Updat Linc01833 drives gemcitabine resistance in non-small cell lung cancer by shielding SLC7A11 from WWP1-mediated ubiquitination and inhibiting ferroptosis. | ||
Cell Mol Biol Lett Extrachromosomal circular DNA promotes prostate cancer progression through the FAM84B/CDKN1B/MYC/WWP1 axis | ||
Free Radic Biol Med RTA-408 overcomes cisplatin-resistant lung cancer by inhibiting WWP1-mediated NCOA4 ubiquitination to induce ferritinophagy and ferroptosis | ||
J Biochem Mol Toxicol COTE1 Regulates AMPKα2 Deubiquitination by Targeting WWP1 Activation to Promote Proliferation and Autophagy in Small Cell Lung Cancer | ||
Cancer Med Quercetin-induced degradation of RhoC suppresses hepatocellular carcinoma invasion and metastasis | ||
Mol Med Targeting Cathepsin B with p-coumaric acid rescues WWP1-dependent proteasomal degradation of NLRP3 in hyperuricemic nephropathy. |
Reviews
What customers say
One reviewer used this antibody for Western Blot on PC3 cells at a 1:1000 dilution and rated it 3 out of 5. The only concern noted was that the background was a little bit strong. Overall, the product performed adequately for its intended application, though the slightly elevated background may warrant optimization of blocking or washing conditions for cleaner results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Xue (Verified Customer) (08-14-2026) | background is a little bit strong
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