Product Information
87251-1-PBS targets XRCC1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag15172 Product name: Recombinant human XRCC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 284-633 aa of BC023593 Sequence: TAPVPARAQGAVTGKPRGEGTEPRRPRAGPEELGKILQGVVVVLSGFQNPFRSELRDKALELGAKYRPDWTRDSTHLICAFANTPKYSQVLGLGGRIVRKEWVLDCHRMRRRLPSQRYLMAGPGSSSEEDEASHSGGSGDEAPKLPQKQPQTKTKPTQAAGPSSPQKPPTPEETKAASPVLQEDIDIEGVQSEGQDNGAEDSGDTEDELRRVAEQKEHRLPPGQEENGEDPYAGSTDENTDSEEHQEPPDLPVPELPDFFQGKHFFLYGEFPGDERRKLIRYVTAFNGELEDYMSDRVQFVITAQEWDPSFEEALMDNPSLAFVRPRWIYSCNEKQKLLPHQLYGVVPQA Predict reactive species |
| Full Name | X-ray repair complementing defective repair in Chinese hamster cells 1 |
| Calculated Molecular Weight | 633 aa, 70 kDa |
| Observed Molecular Weight | 80-90 kDa |
| GenBank Accession Number | BC023593 |
| Gene Symbol | XRCC1 |
| Gene ID (NCBI) | 7515 |
| RRID | AB_3745432 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P18887 |
| Storage Buffer | PBS only, pH 7.3. |
| Storage Conditions | Store at -80°C. |
Background Information
XRCC1 (X-ray Repair Cross Complementing group 1) is a molecular scaffold protein that interacts with multiple enzymatic components of DNA single-strand break repair (SSBR) including DNA kinase, DNA phosphatase, DNA polymerase, DNA deadenylase, and DNA ligase activities that collectively are capable of accelerating the repair of a broad range of DNA single-strand breaks (SSBs) (PMID: 18515191). XRCC1 is 633 amino acids in length which is comprised of an N-terminal domain (NTD) of ˜160 amino acids that interacts with Pol β, a central BRCT domain of ˜90 amino acids that interacts with PARP1 (PMID: 9584196). This antibody is not suitable for murine samples.
