XRCC1 Recombinant monoclonal antibody

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• XRCC1 Recombinant monoclonal antibody (87251-1-RR)

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  WB analysis using 87251-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87251-1-RR (XRCC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 87251-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using XRCC1 antibody (87251-1-RR, Clone: 252518B12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity of 87251-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87251-1-RR against Human XRCC1 were performed. The affinity constant is below 1 pM.

XRCC1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87251-1-RR

Clone No.252518B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

DNA repair protein XRCC1, X-ray repair cross-complementing protein 1

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, K-562 cells, U2OS cells
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87251-1-RR targets XRCC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag15172

  Product name: Recombinant human XRCC1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 284-633 aa of BC023593

  Sequence: TAPVPARAQGAVTGKPRGEGTEPRRPRAGPEELGKILQGVVVVLSGFQNPFRSELRDKALELGAKYRPDWTRDSTHLICAFANTPKYSQVLGLGGRIVRKEWVLDCHRMRRRLPSQRYLMAGPGSSSEEDEASHSGGSGDEAPKLPQKQPQTKTKPTQAAGPSSPQKPPTPEETKAASPVLQEDIDIEGVQSEGQDNGAEDSGDTEDELRRVAEQKEHRLPPGQEENGEDPYAGSTDENTDSEEHQEPPDLPVPELPDFFQGKHFFLYGEFPGDERRKLIRYVTAFNGELEDYMSDRVQFVITAQEWDPSFEEALMDNPSLAFVRPRWIYSCNEKQKLLPHQLYGVVPQA

• Full Name: X-ray repair complementing defective repair in Chinese hamster cells 1
• Calculated Molecular Weight: 633 aa, 70 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC023593
• Gene Symbol: XRCC1
• Gene ID (NCBI): 7515
• RRID: AB_3745432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P18887
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XRCC1 (X-ray Repair Cross Complementing group 1) is a molecular scaffold protein that interacts with multiple enzymatic components of DNA single-strand break repair (SSBR) including DNA kinase, DNA phosphatase, DNA polymerase, DNA deadenylase, and DNA ligase activities that collectively are capable of accelerating the repair of a broad range of DNA single-strand breaks (SSBs) (PMID: 18515191). XRCC1 is 633 amino acids in length which is comprised of an N-terminal domain (NTD) of ˜160 amino acids that interacts with Pol β, a central BRCT domain of ˜90 amino acids that interacts with PARP1 (PMID: 9584196). This antibody is not suitable for murine samples.