ZAP70 Monoklonaler Antikörper

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• KD/KO Validated

ZAP70 Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

CloneNo.

3B3C6

Kat-Nr. : 60200-2-Ig

Synonyme

70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 60200-2-Ig

  WB result of ZAP70 antibody (60200-2-Ig, 1:5000) with si-control and si-ZAP70 transfected Jurkat cells..

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  WB analysis of Jurkat using 60200-2-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-2-Ig (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 60200-2-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-2-Ig (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60200-2-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-2-Ig (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 60200-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 60200-2-Ig(ZAP70 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 60200-2-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 60200-2-Ig(ZAP70 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60200-2-Ig bindet in WB,ELISA ZAP70 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ZAP70 fusion protein Ag7943
• Vollständiger Name: zeta-chain (TCR) associated protein kinase 70kDa
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC039039
• Gene symbol: ZAP70
• Gene ID (NCBI): 7535
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.