ZO1 Rekombinanter Antikörper

ZO1 Rekombinant Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2P13

Kat-Nr. : 82870-1-RR

Synonyme

DKFZp686M05161, Tight junction protein 1, Tight junction protein ZO 1, TJP1, ZO 1, ZO1, Zona occludens protein 1, Zonula occludens protein 1

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Galerie der Validierungsdaten

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  WB analysis using 82870-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82870-1-RR (ZO1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 82870-1-RR

  IP result of anti-ZO1(IP:82870-1-RR, 4ug; Detection:82870-1-RR 1:2000) with HEK-293 cells lysate 920 ug.

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  IF Staining of MCF-7 using 82870-1-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO1 antibody (82870-1-RR, Clone: 2P13 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82870-1-RR bindet in WB, IP, IF, ELISA ZO1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZO1 fusion protein Ag33182
• Vollständiger Name: tight junction protein 1 (zona occludens 1)
• Berechnetes Molekulargewicht: 1748 aa, 195 kDa
• Beobachtetes Molekulargewicht: 230 kDa
• GenBank-Zugangsnummer: BC111712
• Gene symbol: TJP1
• Gene ID (NCBI): 7082
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signaling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).