Anticorps Polyclonal de lapin anti-ADRB2

• Phare
• Validé par KD/KO

ADRB2 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (2)

N° de cat : 13096-1-AP

Synonymes

ADRB2, ADRB2R, ADRBR, B2AR, BAR, Beta 2 adrenergic receptor, Beta 2 adrenoceptor, Beta 2 adrenoreceptor, BETA2AR

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Galerie de données de validation

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  WB analysis of mouse heart using 13096-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13096-1-AP (ADRB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 13096-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 13096-1-AP (ADRB2 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 13096-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13096-1-AP (ADRB2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 13096-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13096-1-AP (ADRB2 Antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of PC-3 using 13096-1-AP

  1X10^6 PC-3 cells were stained with 0.2ug ADRB2 antibody (13096-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat
• Résultats positifs en IHC: tissu cardiaque humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Informations sur le produit

13096-1-AP cible ADRB2 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADRB2 Protéine recombinante Ag3715
• Nom complet: adrenergic, beta-2-, receptor, surface
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC012481
• Symbole du gène: ADRB2
• Identification du gène (NCBI): 154
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Beta-2 adrenergic receptor (ADRB2), a member of the G protein-coupled receptor (GPCR) family, binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. ADRB2 is directly linked to one of its final effectors, the class C L-type Ca2+ channel, Cav1.2 (PMID: 11441182). This receptor-channel complex also contains a G protein, an adenylyl cyclase, cyclic adenosine monophosphate-dependent protein kinase (PKA), and a counteracting phosphatase, PP2A. Different polymorphic forms, point mutations, and/or downregulation of the gene of ADRB2 are associated with nocturnal asthma, obesity and type 2 diabetes. This antibody detects ADRB2 with an apparent molecular weight of 50-55 kDa as has been shown by some researches (PMID: 25348166; 12502786; 29232706; 23984976; 19584355).

Publications

Species	Application	Title
human,mouse	WB,IHC	Drug Des Devel Ther; 10-Gingerol Enhances the Effect of Taxol in Triple-Negative Breast Cancer via Targeting ADRB2 Signaling; Authors - Yuqi Liang ; KD Validated
rat	WB,IHC	Proc Natl Acad Sci U S A; Astrocytic β2-adrenergic receptors mediate hippocampal long-term memory consolidation.; Authors - Virginia Gao
mouse	WB	Diabetes; Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity via Inhibiting USP33-Mediated ADRB2 Deubiquitination.; Authors - Yuguo Niu
rat	WB	Mol Ther Nucleic Acids; Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes.; Authors - Zhijie Shen
human	WB,IF	Int J Mol Sci; β-Adrenoceptor Activation in Breast MCF-10A Cells Induces a Pattern of Catecholamine Production Similar to that of Tumorigenic MCF-7 Cells.; Authors - Filipa Amaro
mouse	WB	Sci Rep; SMN deficiency causes pain hypersensitivity in a mild SMA mouse model through enhancing excitability of nociceptive dorsal root ganglion neurons.; Authors - Ruobing Qu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elisa (Verified Customer) (04-14-2022)

It didn´t work. I used this antibody costained with a marker for microglia (Iba1) and a marker for neuron (neun). I tried two different protocols of immunofluorescence and one of them was with an antigen retrieval step. In both cases I didn´t have luck in obtaining a good result. You can see in the images that I attached.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: tissue, mouse spinal cord

ADRB2 Antibody Immunofluorescence validation (1:50 dilution) in tissue, mouse spinal cord (Cat no:13096-1-AP)

FH

Tongbin (Verified Customer) (08-25-2020)

This antibody works beautifully in western blots.

• Applications: Western Blot
• Cell Tissue Type: Heart

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