Anticorps Monoclonal anti-CD107a / LAMP1
CD107a / LAMP1 Monoclonal Antibody for IF/ICC, FC (Intra)
Hôte / Isotype
Mouse / IgG1, kappa
Réactivité testée
Humain
Applications
IF/ICC, FC (Intra)
Conjugaison
CoraLite®594 Fluorescent Dye
CloneNo.
H4A3
N° de cat : CL594-65051
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en IF/ICC | cellules HeLa, |
| Résultats positifs en FC (Intra) | cellules HeLa |
Dilution recommandée
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
CL594-65051 cible CD107a / LAMP1 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Hôte / Isotype | Mouse / IgG1, kappa |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | Cellules adhérentes humaines de sang périphérique |
| Nom complet | lysosomal-associated membrane protein 1 |
| Masse moléculaire calculée | 45 kDa |
| Numéro d’acquisition GenBank | BC006345 |
| Symbole du gène | LAMP1 |
| Identification du gène (NCBI) | 3916 |
| Conjugaison | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Forme | Liquide |
| Méthode de purification | Purification par protéine G |
| Tampon de stockage | PBS with 0.09% sodium azide and 0.5% BSA |
| Conditions de stockage | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Informations générales
LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).
Protocole
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 CD107a / LAMP1 antibody CL594-65051 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |

