Anticorps Monoclonal anti-CD107a / LAMP1

CD107a / LAMP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

H4A3

N° de cat : CL594-65051

Synonymes

CD107a, LAMP1, CD107 antigen-like family member A, H4A3, LAMP 1

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Galerie de données de validation

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  IF Staining of HeLa using CL594-65051

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated CD107a antibody (CL594-65051, Clone: H4A3) at dilution of 1:400. F-actin was stained using CL488-phalloidin (green) and DNA was stained by DAPI (blue).

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  FC experiment of HeLa using CL594-65051

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CD107a / LAMP1 (CL594-65051, Clone:H4A3)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-65051 cible CD107a / LAMP1 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules adhérentes humaines de sang périphérique
• Nom complet: lysosomal-associated membrane protein 1
• Masse moléculaire calculée: 45 kDa
• Numéro d’acquisition GenBank: BC006345
• Symbole du gène: LAMP1
• Identification du gène (NCBI): 3916
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

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