Anticorps Monoclonal anti-CD107a / LAMP1

CD107a / LAMP1 Monoclonal Antibody for IF/ICC, FC, FC (Intra)

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

IF/ICC, FC, FC (Intra)

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

H4A3

N° de cat : FITC-65051

Synonymes

CD107a, LAMP1, LAMP-1, LAMP 1, H4A3

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Galerie de données de validation

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  IF Staining of HeLa using FITC-65051

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FITC-65051 (CD107a antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using FITC-65051

  1x10^6 HeLa cells were intracellularly stained with 10 ul FITC Plus Anti-Human CD107a / LAMP1 (H4A3) (FITC-65051, Clone:H4A3)(red), or 10 ul FITC Plus Mouse IgG1 Isotype Control (MOPC-21) (FITC-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of human PBMCs using FITC-65051

  1X10^6 PHA-activated human PBMCs were surface stained with 5 ul FITC Plus Plus Anti-Human CD107a (FITC-65051, Clone: H4A3). Unactivated PBMCs were used as negative control. Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using FITC-65051

  1X10^6 PHA-activated human PBMCs were surface stained with 5 ul FITC Plus Plus Anti-Human CD107a (FITC-65051, Clone: H4A3). Unactivated PBMCs were used as negative control. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,
• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65051 cible CD107a / LAMP1 dans les applications de IF/ICC, FC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules adhérentes humaines de sang périphérique
• Nom complet: lysosomal-associated membrane protein 1
• Masse moléculaire calculée: 45 kDa
• Numéro d’acquisition GenBank: BC006345
• Symbole du gène: LAMP1
• Identification du gène (NCBI): 3916
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

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