Anticorps Recombinant de lapin anti-CD107b / LAMP2

CD107b / LAMP2 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

H4B4

N° de cat : 65520-1-PBS

Synonymes

CD107b, LAMP2, CD107 antigen-like family member B, H4B4, LAMP 2

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Galerie de données de validation

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  FC experiment of human PBMCs using 65520-1-PBS

  1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-PBS, Clone:H4B4) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

• 

  FC experiment of human PBMCs using 65520-1-RR (same clone as 65520-1-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-RR, Clone:H4B4) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining. This data was developed using the same antibody clone with 65520-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65520-1-RR (same clone as 65520-1-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-RR, Clone:H4B4) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining. This data was developed using the same antibody clone with 65520-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65520-1-PBS

  1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-PBS, Clone:H4B4) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

• 

  FC experiment of human PBMCs using 65520-1-PBS

  1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-PBS, Clone:H4B4) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000 (left) or 0.2 ug Anti-Human CD107b Mouse Monoclonal Antibody (Clone:H4B4) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (center and right). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

• 

  FC experiment of human PBMCs using 65520-1-RR (same clone as 65520-1-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD107b Rabbit Recombinant Antibody (65520-1-RR, Clone:H4B4) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000 (left) or 0.2 ug Anti-Human CD107b Mouse Monoclonal Antibody (Clone:H4B4) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (center and right). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining. This data was developed using the same antibody clone with 65520-1-PBS in a different storage buffer formulation.

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Applications testées

• Résultats positifs en FC (Intra): CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65520-1-PBS cible CD107b / LAMP2 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Cellules adhérentes humaines de sang périphérique
• Nom complet: lysosomal-associated membrane protein 2
• Masse moléculaire calculée: 45 kDa
• Numéro d’acquisition GenBank: BC002965
• Symbole du gène: LAMP2
• Identification du gène (NCBI): 3920
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. Stable for one year after shipment.

Informations générales

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

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