Anticorps Monoclonal anti-CD107b / LAMP2

CD107b / LAMP2 Monoclonal Antibody for IF/ICC, FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

IF/ICC, FC

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

H4B4

N° de cat : FITC-65053

Synonymes

LAMP2, CD107b, H4B4, LAMP 2, CD107 antigen-like family member B, LAMP-2, LAMPB, LGP110, LGP-96, Lysosome-associated membrane glycoprotein 2, Lysosome-associated membrane protein 2

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Galerie de données de validation

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  IF Staining of HeLa using FITC-65053

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FITC-65053 (CD107b antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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  FC experiment of human PBMCs using FITC-65053

  1X10^6 PHA-activated human PBMCs were surface stained with 5 ul FITC Plus Plus Anti-Human CD107b (FITC-65053, Clone: H4B4). Unactivated PBMCs were used as negative control. Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using FITC-65053

  1X10^6 PHA-activated human PBMCs were surface stained with 5 ul FITC Plus Plus Anti-Human CD107b (FITC-65053, Clone: H4B4). Unactivated PBMCs were used as negative control. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65053 cible CD107b / LAMP2 dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules adhérentes humaines de sang périphérique
• Nom complet: lysosomal-associated membrane protein 2
• Masse moléculaire calculée: 45 kDa
• Numéro d’acquisition GenBank: BC002965
• Symbole du gène: LAMP2
• Identification du gène (NCBI): 3920
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

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