Anticorps Monoclonal anti-CD117/c-Kit

CD117/c-Kit Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

bovin, Humain

Applications

FC

Conjugaison

APC Fluorescent Dye

CloneNo.

104D2

N° de cat : APC-65154

Synonymes

CD117, KIT, 104D2, C Kit, c-Kit

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Galerie de données de validation

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  FC experiment of TF-1 using APC-65154

  1X10^6 TF-1 cells were surface stained with 10.00 ul/test APC Anti-Human CD117 (APC-65154, Clone:104D2) (red) or isotype control antibody (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules TF-1

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-65154 cible CD117/c-Kit dans les applications de FC et montre une réactivité avec des échantillons bovin, Humain

• Réactivité: bovin, Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules mégacaryocytaires MOLM-1
• Nom complet: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Masse moléculaire calculée: 976 aa, 110 kDa
• Numéro d’acquisition GenBank: BC071593
• Symbole du gène: CD117
• Identification du gène (NCBI): 3815
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: L'anticorps purifié est conjugué à l'allophycocyanine (APC) dans des conditions optimales. Le conjugué est purifié par chromatographie d'exclusion de taille.
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

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