Anticorps Monoclonal anti-CD117

CD117 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

bovin, Humain

Applications

FC

Conjugaison

PE Fluorescent Dye

CloneNo.

104D2

N° de cat : PE-65154

Synonymes

C Kit, CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit



Applications testées

Résultats positifs en cytométriecellules TF-1

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 20 µl per 10^6 cells in a 100 µl suspension or 20 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-65154 cible CD117 dans les applications de FC et montre une réactivité avec des échantillons bovin, Humain

Réactivité bovin, Humain
Hôte / Isotype Mouse / IgG1, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène Cellules mégacaryocytaires MOLM-1
Nom complet v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Masse moléculaire calculée 976 aa, 110 kDa
Numéro d’acquisition GenBankBC071593
Symbole du gène KIT
Identification du gène (NCBI) 3815
Conjugaison PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Forme Liquide
Méthode de purification L'anticorps purifié est conjugué à la R-phycoérithrine (PE) dans des conditions optimales. Le conjugué est purifié par chromatographie d'exclusion de taille.
Tampon de stockage PBS avec azoture de sodium à 0,1 %.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

Protocole

Product Specific Protocols
FC protocol for PE CD117 antibody PE-65154Download protocol
Standard Protocols
Click here to view our Standard Protocols