Anticorps Monoclonal anti-CD11b

CD11b Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

Humain, souris

Applications

IF, FC

Conjugaison

FITC Plus Fluorescent Dye

Publications

8

CloneNo.

M1/70

N° de cat : FITC-65055

Synonymes

CD11b, CD11b/CD18, CR3, CR3A, F730045J24Rik, integrin alpha M, Itgam, Ly 40, Mac 1, Mac 1a, MAC1

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Galerie de données de validation

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  FC experiment of Balb/c mouse bone marrow cells using FITC-65055

  1X10^6 Balb/c mouse bone marrow cells were surface co-stained with 0.25 ug APC Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5) and 0.25 ug FITC Plus Anti-Mouse CD11b (FITC-65055, Clone: M1/70) or 0.25 ug FITC Plus Rat IgG2b Isotype Control (LTF-2). Cells were not fixed.

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  FC experiment of human PBMCs using FITC-65055

  1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Mouse CD11b (FITC-65055, Clone: M1/70). Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65055

  1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Mouse CD11b (FITC-65055, Clone:M1/70). Cells were not fixed. Monocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: Balb/c mouse bone marrow cells, CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below
• FC: See 6 publications below

Informations sur le produit

FITC-65055 cible CD11b dans les applications de IF, FC et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes T spléniques de souris C57BL/10 et splénocytes C57BL/10 activés par la concanavaline A
• Nom complet: integrin alpha M
• Numéro d’acquisition GenBank: BC156991
• Symbole du gène: Itgam
• Identification du gène (NCBI): 16409
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Publications

Species	Application	Title
		Cell Chem Biol; Small-molecule MHC-II inducers promote immune detection and anti-cancer immunity via editing cancer metabolism; Authors - Ling Huang
mouse	IF,FC	Int Immunopharmacol; Methionine enkephalin inhibited cervical carcinoma via apoptosis promotion and reduction of myeloid derived suppressor cell infiltrated in tumor.; Authors - Na Qu
mouse	FC	Cell Death Dis; Lactate secreted by PKM2 upregulation promotes Galectin-9-mediated immunosuppression via inhibiting NF-κB pathway in HNSCC.; Authors - Hanyue Chang
mouse	FC	Cell Death Discov; TRAF6 triggers Mycobacterium-infected host autophagy through Rab7 ubiquitination; Authors - Qinmei Ma
mouse	FC	Bioact Mater; Targeted transplantation of engineered mitochondrial compound promotes functional recovery after spinal cord injury by enhancing macrophage phagocytosis; Authors - Jiaqi Xu
mouse	IF	Molecules; Tabersonine Inhibits the Lipopolysaccharide-Induced Neuroinflammatory Response in BV2 Microglia Cells via the NF-κB Signaling Pathway; Authors - Jiaotai Shi