Anticorps Monoclonal anti-CD14

CD14 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

UCHM-1

N° de cat : CL594-65056

Synonymes

CD14 molecule, Monocyte differentiation antigen CD14, membrane-bound form, Monocyte differentiation antigen CD14, urinary form, Myeloid cell-specific leucine-rich glycoprotein, UCHM-1

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Galerie de données de validation

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  FC experiment of human PBMCs using CL594-65056

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD14 (CL594-65056, Clone:UCHM-1) or CoraLite®594 Mouse IgG2a Isotype Control (CL594-65208, Clone: C1.18.4). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using CL594-65056

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD14 (CL594-65056, Clone:UCHM-1) or CoraLite®594 Mouse IgG2a Isotype Control (CL594-65208, Clone: C1.18.4). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-65056 cible CD14 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Thymocytes et lymphocytes de sang périphérique d'un patient en rémission d'une LAM
• Nom complet: CD14 molecule
• Masse moléculaire calculée: 375 aa, 40 kDa
• Numéro d’acquisition GenBank: BC010507
• Symbole du gène: CD14
• Identification du gène (NCBI): 929
• Conjugaison: CoraLite® Plus 594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 594 nm / 615 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

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