CoraLite® Plus 405 Anti-Human CD14 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using CL405-98040

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD11b, and 5 ul CoraLite® Plus 405 Anti-Human CD14 Rabbit RecAb (CL405-98040, Clone: 230332D7) or CoraLite® Plus 405 Rabbit IgG Isotype Control RecAb (CL405-98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of human PBMCs using CL405-98040

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD14 Rabbit RecAb (CL405-98040, Clone: 230332D7) (red) or CoraLite® Plus 405 Rabbit IgG Isotype Control RecAb (CL405-98136, Clone: 240953C9) (blue). Cells were not fixed.

CD14 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL405-98040

Clone No.230332D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

230332D7, CD14 molecule, Monocyte differentiation antigen CD14, membrane-bound form, Monocyte differentiation antigen CD14, urinary form, Myeloid cell-specific leucine-rich glycoprotein

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL405-98040 targets CD14 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD14 fusion protein Eg0498
• Full Name: CD14 molecule
• Calculated Molecular Weight: 375 aa, 40 kDa
• GenBank Accession Number: BC010507
• Gene Symbol: CD14
• Gene ID (NCBI): 929
• ENSEMBL Gene ID: ENSG00000170458
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08571
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.