Anticorps Monoclonal anti-CD16

CD16 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / Mouse IgG1, k

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

3G8

N° de cat : CL405-65090

Synonymes

CD16, CD16A, CD16a antigen, Fc gamma RIII, Fc gamma RIII alpha, Fc gamma RIIIa, FCG3, FCGR3, FCGR3A, FCGRIII, FCR 10, FCRIII, FCRIIIA, IGFR3, IgG Fc receptor III 2



Applications testées

Résultats positifs en cytométrieCMSP humaines,

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL405-65090 cible CD16 dans les applications de FC et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / Mouse IgG1, k
Clonalité Monoclonal
Type Anticorps
Immunogène s/o
Nom complet Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Masse moléculaire calculée 254 aa, 29 kDa
Numéro d’acquisition GenBankBC017865
Symbole du gène FCGR3A
Identification du gène (NCBI) 2214
Conjugaison CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission maxima wavelengths399 nm / 422 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS with 0.09% sodium azide and 0.5% BSA.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. Clone 3G8 recognizes both the CD16a and CD16b (PMID: 7592758).

Protocole

Product Specific Protocols
FC protocol for CL Plus 405 CD16 antibody CL405-65090Download protocol
Standard Protocols
Click here to view our Standard Protocols