Anticorps Polyclonal de lapin anti-CD1e

CD1e Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 22463-1-AP

Synonymes

R2G1, R2, hCD1e, CD1e molecule

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Galerie de données de validation

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  WB analysis using 22463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1e antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 22463-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 22463-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 22463-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 22463-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22463-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22463-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules MOLT-4

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

22463-1-AP cible CD1e dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD1e Protéine recombinante Ag18008
• Nom complet: CD1e molecule
• Masse moléculaire calculée: 388 aa, 44 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC131693
• Symbole du gène: CD1E
• Identification du gène (NCBI): 913
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD1e molecule (CD1e, also known as R2 and CD1A) is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin (PMID: 10948205). The discoverers of the CD1 locus originally separated CD1 proteins into group 1 (CD1a, CD1b, and CD1c) and group 2 (CD1d), based on amino acid sequence homology (PMID: 2467814). CD1e is an important modulator of group 1 and group 2 CD1-restricted responses influencing the lipid antigen availability and the generation and persistence of CD1-lipid complexes (PMID: 21844346).

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