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Anticorps Polyclonal de lapin anti-CD27

CD27 Polyclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

N° de cat : 11121-1-AP

Synonymes

CD27, CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27, T14, TNFRSF7, Tp55

Galerie de données de validation

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  • Western Blot (WB) analysis of Raji cells using CD27 Polyclonal antibody (11121-1-AP)

    WB analysis of Raji using 11121-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 11121-1-AP (CD27 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Raji cells using CD27 Polyclonal antibody (11121-1-AP)

    FC experiment of Raji using 11121-1-AP

    1X10^6 Raji cells were stained with 0.2ug CD27 antibody (11121-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Applications testées

Résultats positifs en WBcellules Raji,
Résultats positifs en cytométriecellules Raji

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

11121-1-AP cible CD27 dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène CD27 Protéine recombinante Ag1582
Nom complet CD27 molecule
Masse moléculaire calculée 29 kDa
Poids moléculaire observé 45-50 kDa
Numéro d’acquisition GenBankBC012160
Symbole du gène CD27
Identification du gène (NCBI) 939
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

Protocole

Product Specific Protocols
WB protocol for CD27 antibody 11121-1-APDownload protocol
FC protocol for CD27 antibody 11121-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols