Anticorps Monoclonal anti-PD-1/CD279

PD-1/CD279 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC

Conjugaison

PE Fluorescent Dye

CloneNo.

J110

N° de cat : PE-65119

Synonymes

CD279, hPD 1, hPD l, PD1, PD-1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2



Applications testées

Résultats positifs en cytométrielymphocytes de sang périphérique humain,

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-65119 cible PD-1/CD279 dans les applications de FC et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène s/o
Nom complet programmed cell death 1
Masse moléculaire calculée 288 aa, 32 kDa
Numéro d’acquisition GenBankBC074740
Symbole du gène PDCD1
Identification du gène (NCBI) 5133
Conjugaison PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

Protocole

Product Specific Protocols
FC protocol for PE PD-1/CD279 antibody PE-65119Download protocol
Standard Protocols
Click here to view our Standard Protocols