Anticorps Monoclonal anti-CD4

CD4 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2b, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

Publications(2)

CloneNo.

OKT4

N° de cat : FITC-65134

Synonymes

CD4 molecule, CD4mut, OKT4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

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Galerie de données de validation

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  FC experiment of human PBMCs using FITC-65134

  1X10^6 human PBMCs were surface co-stained with Anti-Human CD3 and 5 ul FITC Plus Anti-Human CD4 (FITC-65134, Clone:OKT4), or 5 ul Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65134

  1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD4 (FITC-65134, Clone:OKT4), or 5 ul Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 2 publications below

Informations sur le produit

FITC-65134 cible CD4 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes T périphériques humains
• Nom complet: CD4 molecule
• Masse moléculaire calculée: 55 kDa
• Numéro d’acquisition GenBank: BC025782
• Symbole du gène: CD4
• Identification du gène (NCBI): 920
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Publications

Species	Application	Title
	FC	Pharmacol Res; CX-5461 is a potent immunosuppressant which inhibits T cell-mediated alloimmunity via p53-DUSP5.; Authors - Guopin Pan
human	FC	Autoimmunity; N6-methyladenosine modification of THBS1 induced by affluent WTAP promotes Graves' ophthalmopathy progression through glycolysis to affect Th17/Treg balance; Authors - Lin-Na Li

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