Anticorps Monoclonal anti-CD45.2

CD45.2 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2a, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

APC Fluorescent Dye

CloneNo.

104

N° de cat : APC-65072

Synonymes

B220, CD45.2, CD45R, loc, Ly 5, Lyt 4, Ptprc, T200



Applications testées

Résultats positifs en cytométriesplénocytes de souris

Dilution recommandée

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-65072 cible CD45.2 dans les applications de FC et montre une réactivité avec des échantillons souris

Réactivité souris
Hôte / Isotype Mouse / IgG2a, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène Thymocytes et splénocytes de souris B10.S
Nom complet protein tyrosine phosphatase, receptor type, C
Numéro d’acquisition GenBankBC028512
Symbole du gène Ptprc
Identification du gène (NCBI) 19264
Conjugaison APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage Tampon à base de phosphate avec azoture de sodium à 0,09 % et gélatine à 0,1 %, pH 7,2.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673).

Protocole

Product Specific Protocols
FC protocol for APC CD45.2 antibody APC-65072Download protocol
Standard Protocols
Click here to view our Standard Protocols