CoraLite® Plus 647 Anti-Mouse CD45.2 Rabbit Recombinant Antibody

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  FC experiment of C57BL/6 Mouse Splenocytes using CL647-98168

  1x10^6 C57BL/6 mouse splenocytes (left) or SJL mouse splenocytes (right) were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD45.2 Rabbit Recombinant Antibody (CL647-98168, Clone:240922E12)(red) or CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

CD45.2 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98168

Clone No.240922E12

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Cd45, Ptprc, L-CA, LCA, EC:3.1.3.48

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Tested Applications

• Positive FC detected in: C57BL/6 mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98168 targets CD45.2 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein tyrosine phosphatase, receptor type, C
• Gene Symbol: CD45
• Gene ID (NCBI): 19264
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673).