Anticorps Monoclonal anti-CD45

CD45 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

IF, FC

Conjugaison

PE Fluorescent Dye

Publications(1)

CloneNo.

2D1

N° de cat : PE-65082

Synonymes

2D1, EC:3.1.3.48, GP180, L CA, LCA

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Galerie de données de validation

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  FC experiment of human peripheral blood lymphocytes using PE-65082

  1X10^6 human peripheral blood lymphocytes were surface stained with 5 ul PE-Anti-Human CD45 (PE-65082, clone 2D1) (red) or PE-mouse IgG1 isotype control (blue). Cells were not fixed.

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  FC experiment of Raji using PE-65082

  1X10^6 Raji cells were surface stained with 5 ul PE Anti-Human CD45 (PE-65082, Clone:2D1) (red), or 5 ul Control Antibody. Cells were not fixed .

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Applications testées

• Résultats positifs en cytométrie: lymphocytes de sang périphérique humain, cellules Raji

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

PE-65082 cible CD45 dans les applications de IF, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CMSP humaines
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Numéro d’acquisition GenBank: BC014239
• Symbole du gène: CD45
• Identification du gène (NCBI): 5788
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.1% gelatin
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Publications

Species	Application	Title
human	IF	Exp Ther Med; Detection of PD‑L1 expression and epithelial‑mesenchymal transition of circulating tumor cells in non‑small cell lung cancer; Authors - Jianping Jiang

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