Anticorps Monoclonal anti-CD54

CD54 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

YN1/1.7.4

N° de cat : 65066-1-Ig

Synonymes

CD54, ICAM 1, Icam1, Ly 47, MALA 2

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 65066-1-Ig

  1X10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD54 (65066-1-Ig, Clone: YN1/1.7.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:200 (green), or stained with 0.5 ug Rat IgG2b Isotype Control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:200 (black). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65066-1-Ig cible CD54 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules NS-1 de souris
• Nom complet: intercellular adhesion molecule 1
• Numéro d’acquisition GenBank: BC008626
• Symbole du gène: Icam1
• Identification du gène (NCBI): 15894
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Solution saline tamponnée au borate, pH 8,2.
• Conditions de stockage: Stocker à une température comprise entre 2 °C et 8 °C.

Informations générales

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).