CoraLite® Plus 750 Anti-Mouse ICAM-1/CD54 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using CL750-98049

  1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD3, and 0.25 ug CoraLite® Plus 750 Anti-Mouse ICAM-1/CD54 Rabbit Recombinant Antibody (CL750-98049, Clone: 240249D10) or Isotype Control. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL750-98049

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 750 Anti-Mouse ICAM-1/CD54 Rabbit Recombinant Antibody (CL750-98049, Clone: 240249D10) (red) or 0.25 ug Isotype Control (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

ICAM-1/CD54 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL750-98049

Clone No.240249D10

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD54, Icam-1, 240249D10, ICAM 1, Icam1

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-98049 targets ICAM-1/CD54 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ICAM-1/CD54 fusion protein Eg0482
• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 59 kDa
• GenBank Accession Number: NM_010493
• Gene Symbol: ICAM-1
• Gene ID (NCBI): 15894
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13597
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).