Anticorps Monoclonal anti-CD63

CD63 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

H5C6

N° de cat : 65295-1-Ig

Synonymes

Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, H5C6, CD63 antigen

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Galerie de données de validation

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  FC experiment of human peripheral blood platelets using 65295-1-Ig

  100ul thrombin-activated (red) or unactivated (black dashed) human peripheral blood platelets (diluted 1:2 in PBS) were surface stained with 0.2 ug Anti-Human CD63 (65295-1-Ig, Clone: H5C6) or Mouse IgG1 Isotype Control (65124-1-Ig) (blue) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed.

• 

  FC experiment of human peripheral blood platelets using 65295-1-Ig

  100ul thrombin-activated (red) or unactivated (black dashed) human peripheral blood platelets (diluted 1:2 in PBS) were surface stained with 0.2 ug Anti-Human CD63 (65295-1-Ig, Clone: H5C6) or Mouse IgG1 Isotype Control (65124-1-Ig) (blue) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: thrombin-activated human peripheral blood platelets

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65295-1-Ig cible CD63 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Human splenic adherent cells
• Nom complet: CD63 molecule
• Numéro d’acquisition GenBank: BC013017
• Symbole du gène: CD63
• Identification du gène (NCBI): 967
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.

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