Anticorps Monoclonal anti-CD68

CD68 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG2b, kappa

Réactivité testée

Humain

Applications

IF, FC (Intra)

Conjugaison

PE Fluorescent Dye

Publications(4)

CloneNo.

Y1/82A

N° de cat : PE-65187

Synonymes

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

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Galerie de données de validation

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  FC experiment of human PBMCs using PE-65187

  1X10^6 human PBMCs were intracellularly stained with 5 ul PE Anti-Human CD68 (PE-65187, Clone:Y1/82A) or unstained. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

• 

  FC experiment of human PBMCs using PE-65187

  1X10^6 human PBMCs were intracellularly stained with 5 ul PE Anti-Human CD68 (PE-65187, Clone:Y1/82A) or unstained. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

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Applications testées

• Résultats positifs en FC (Intra): CMSP humaines
• Résultats positifs en cytométrie: CMSP humaines

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below
• FC: See 1 publications below

Informations sur le produit

PE-65187 cible CD68 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Contenu lysosomal des macrophages pulmonaires
• Nom complet: CD68 molecule
• Masse moléculaire calculée: 37 kDa
• Numéro d’acquisition GenBank: BC015557
• Symbole du gène: CD68
• Identification du gène (NCBI): 968
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: L'anticorps purifié est conjugué à la R-phycoérithrine (PE) dans des conditions optimales. Le conjugué est purifié par chromatographie d'exclusion de taille.
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Publications

Species	Application	Title
	FC (Intra)	Cancer Sci; Homeobox A7 promotes esophageal squamous cell carcinoma progression through C-C motif chemokine ligand 2-mediated tumor-associated macrophage recruitment; Authors - Anqi Feng
human	IF	Sci Adv; Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis; Authors - Shuwei Hu
human	FC	Int Immunopharmacol; OTUD4-mediated inhibition of YAP1 signaling pathway in ovarian cancer: Implications for macrophage polarization and recruitment; Authors - Mingyue Li
human	IF	Am J Cancer Res; FTO-mediated m6A modification of QPCT promotes tumorigenesis in lung adenocarcinoma by inducing macrophage chemotaxis and M2 polarization; Authors - Benkun Liu

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