Anticorps Monoclonal anti-CD69

CD69 Monoclonal Antibody for FC

Hôte / Isotype

Armenian Hamster / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

APC Fluorescent Dye

Publications(1)

CloneNo.

H1.2F3

N° de cat : APC-65105

Synonymes

VEA, H1.2F3, CD69 antigen, AIM

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Galerie de données de validation

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  FC experiment of mouse splenocytes using APC-65105

  1x10^6 untreated or anti-CD3/CD28 treated (2 days) mouse splenocytes were surface stained with 0.2 ug APC Anti-Mouse CD69 (H1.2F3) (APC-65105, Clone: H1.2F3) and PE Anti-Mouse CD3 (17A2) (PE-65077, Clone: 17A2). Cells were not fixed.

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  FC experiment of mouse splenocytes using APC-65105

  1x10^6 anit-CD3/CD28 treated (2 days) mouse splenocytes were surface stained with 0.2 ug APC Anti-Mouse CD69 (H1.2F3) (APC-65105, Clone: H1.2F3) (red) or 0.2 ug APC Armenian Hamster IgG Isotype Control (PIP) (APC-65210, Clone: PIP) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.2 ug APC Anti-Mouse CD69 (H1.2F3) (APC-65105, Clone: H1.2F3) (black, dashed). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: anti-CD3/CD28 treated 2days mouse splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 1 publications below

Informations sur le produit

APC-65105 cible CD69 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Armenian Hamster / IgG
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lignée de lymphocytes T épidermiques dendritiques de souris Y245
• Nom complet: CD69 antigen
• Numéro d’acquisition GenBank: BC106997
• Symbole du gène: Cd69
• Identification du gène (NCBI): 12515
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).

Publications

Species	Application	Title
mouse	FC	Sci Adv; Nutrient availability regulates the secretion and function of immune cell-derived extracellular vesicles through metabolic rewiring; Authors - Yizhuo Wang

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