Anticorps Monoclonal anti-CD86

CD86 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

Publications(4)

CloneNo.

GL1

N° de cat : 65068-1-Ig

Synonymes

B7, B7 2, B7.2, B70, B7-2

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 65068-1-Ig

  1x10^6 unstimulated or LPS-stimulated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD86 (GL1) (65068-1-Ig, Clone: GL1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: LPS treated mouse splenocytes,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 4 publications below

Informations sur le produit

65068-1-Ig cible CD86 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes B de rate de souris CBA/Ca activées par LPS
• Nom complet: CD86 antigen
• Numéro d’acquisition GenBank: BC013807
• Symbole du gène: Cd86
• Identification du gène (NCBI): 12524
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Publications

Species	Application	Title
mouse	FC	Inflammation; Moxibustion Alleviates Inflammation via SIRT5-mediated Post-translational Modification and Macrophage Polarization; Authors - Chuan-Yi Zuo
mouse	FC	MedComm (2020); Full-active pharmaceutical ingredient nanosensitizer for augmented photoimmunotherapy by synergistic mitochondria targeting and immunogenic death inducing; Authors - Xianghui Li
mouse	FC	Int J Biol Macromol; Three polysaccharides from Roughhaired Holly Root with immune-enhancing activity via activation of TLR receptor; Authors - Rong Huang
mouse	FC	Int J Biol Sci; Mannose Inhibits NSCLC Growth and Inflammatory Microenvironment by Regulating Gut Microbiota and Targeting OGT/hnRNP R/JUN/IL-8 Axis; Authors - Haoyi Jin

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