Anticorps Monoclonal anti-CD86

CD86 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

IF, FC

Conjugaison

APC Fluorescent Dye

Publications(14)

CloneNo.

GL1

N° de cat : APC-65068

Synonymes

B7, B7 2, B7.2, B70, B7-2

---

Galerie de données de validation

• 

  FC experiment of mouse splenocytes using APC-65068

  1X10^6 untreated (dashed lines) or LPS-treated (red) mouse splenocytes were surface stained with 0.2 ug APC Anti-Mouse CD86 (APC-65068, Clone:GL1) (red), or 0.2 ug Isotype Control (blue). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using APC-65068

  1X10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.2 ug APC Anti-Mouse CD86 (APC-65068, Clone:GL1). Cells were not fixed.

---

Applications testées

• Résultats positifs en cytométrie: LPS treated mouse splenocytes,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below
• FC: See 11 publications below

Informations sur le produit

APC-65068 cible CD86 dans les applications de IF, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes B de rate de souris CBA/Ca activées par LPS
• Nom complet: CD86 antigen
• Numéro d’acquisition GenBank: BC013807
• Symbole du gène: Cd86
• Identification du gène (NCBI): 12524
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Publications

Species	Application	Title
mouse	FC	Acta Pharm Sin B; Novel Pt(IV) complex OAP2 induces STING activation and pyroptosis via mitochondrial membrane remodeling for synergistic chemo-immunotherapy; Authors - Renming Fan
mouse	FC	ACS Appl Mater Interfaces; Intelligent Nanoplatform Integrating Macrophage and Cancer Cell Membrane for Synergistic Chemodynamic/Immunotherapy/Photothermal Therapy of Breast Cancer; Authors - Shichao Zhang
mouse	IF	J Neuroinflammation; The BET PROTAC inhibitor dBET6 protects against retinal degeneration and inhibits the cGAS-STING in response to light damage; Authors - Xingfei Zhu
mouse	FC	Cell Commun Signal; Transfer of cGAMP from neuron to microglia activates microglial type I interferon responses after subarachnoid hemorrhage; Authors - Hanxiao Chang
mouse	FC	Biomed Pharmacother; Resveratrol alleviated neuroinflammation induced by pseudorabies virus infection through regulating microglial M1/M2 polarization; Authors - Xiangxiu Chen
mouse	FC	Stem Cells Dev; Deer antler reserve mesenchyme cell-conditioned medium reduces the destruction of periodontitis in mice; Authors - Hongbing Lin

{{ptg:RelatedPrimaryAntibodies}}